Api 4000 qtrap manual

Used applied biosystems api 4000 qtrap spectrophotometer. By jianjun chen double click on manual tuning to reopen the tune method editor window. Api 4000 qtrap spectralab scientific inc the api 4000 qtrap lcmsms system is premier hybrid triple quadrupolelinear ion trap mass spectrometer. Just activate your instrument in ms only mode, go to manual tuning and choose q1 scan no collision cell used. On the navigation bar, under tune, doubleclick manual tuning. Enable highsensitivity, fullscan ms, msms, and ms3 spectra. It will be primarily used for quantitative bioanalysis of small and large molecules. The 4000 qtrap system is best suited to three specific applications. This instrument relies on the iondrive technology that provides high sensitivity. Applied biosystems api 4000 qtrap lcmsms 2007 system available for sale.

You can use the procedures to learn how to use the software and the instrument. This instrument is a great addition to our existing ab sciex api 4000 qtrap lcmsms and sciex 5600 triple tofms. We have 1 ab sciex 4000 series manual available for free pdf download. About this manual the api 4000 lc msms system hardware manual is targeted to operators who are familiar with mass spectrometry but are new to the api 4000 lcmsms system. Hi, the turbo pumps on our 4000 qtrap have shut down twice over the last couple of weeks, and we are trying to figure out why. Abi mds sciex 018444 api 3000 triple quad mass spectrometer lcms pred. This easy to use lcms has high sensitivity thanks to the ionization and the interface ion sampling, giving you low detection limits. The system is ideal for laboratories requiring ultimate robustness, ruggedness, and reliability for highthroughput screening of many. This refurbished ab sciex api 4000 lcmsms triple quad is rugged, reliable, and easytouse.

An item that has been restored to working order by the ebay seller or a third party not approved by the manufacturer. Manual optimization is used to optimize compound and ion sourcedependent parameters for. The sciex api 4000 qtrap mass spectrometer system delivers highperformance quantitation and identification of small molecules, peptides, and proteins across a wide range of lc flow rates. Applied biosystems api 4000 qtrap mass spectrometer w shimadzu lc10ad and ctc autosampler. View online or download ab sciex api 5000 lcms ms hardware manual. From discovery to clinical research this system offers. Ab sciex 4000 series site planning manual pdf download. Acquiring data in manual tune mode in analyst software youtube. Mass spec, service manuals andor service information. The api 4000 lcmsms system hardware manual is targeted to operators who are familiar with mass spectrometry but are new to the api 4000 lcmsms system.

Its described in one of the manuals but it requires some caution. Electron multipliers for sciex mass spectrometers api iii lcms elan 5000a elan 250 elan 500 elan 5000 elan 6000 6000a 6100 ap 100, 200, 300, 3000, 4000, 5000 api 2000 4000 qtrap catalog page a43 catalog page a44 catalog page a45 catalog page b26. Ideal for drug discovery and development, metabolite identification, contaminant testing, and proteomic applications including posttranslationally modified protein discovery, protein identification, and biomarker validation. Ab sciex api 4000 lcmsms triple quad cables and manuals proven curtain gas interface technology analyst data system turbov. Ab sciex api 4000 qtrap w shimadzu 20ad uplc lcmsms q. The ab sciex 4500 lcmsms is the intelligently reengineered design of the 4000 qtrap platform and sets a new benchmark for robust quantitation and library searching. Texa marine dealer level tool walkthrough on 2014 mercury 50hp outboard duration. The 4000 qtrap system includes a hybrid triple quadrupolelit linear ion trap.

Instrument frontend cleaning procedure manual sciex. System user guide 4500 series of instruments ruoidv050264a 3 of 8 revision log revision description date d5030863 a first release of document. Applied biosystems api 4000 qtrap mass spectrometer w. Site planning guide ab sciex 4000 series of instruments ruoidv010563a d5040008 a 33 of 33. Api 4000 qtrap triple quadrupole linear ion trap lcmsms sensitivity for detection of lowlevel metabolites. This document combines the api 4000 june 2014 lcmsms system site planning guide pn 1041973a and the 4000 qtrap lcmsms system site planning guide pn 1042287a. The scientists choice award for best new spectroscopy product awarded to the ab sciex qtrap r 5500 system from applied biosystemsmds analytical technologies. The 4000 qtrap lcmsms system is our premier hybrid triple quadrupolelinear ion trap mass spectrometer. You should this manual contains the instructions required to start up, calibrate, optimize, for api 365, api 3000, and api 4000 instruments, turbo gas flows of about.

Api 3200 and 3200 qtrap systems 50 ngml api 4000 and 4000 qtrap systems 10 ngml api 5000 system 10 ngml ab sciex qtrap 5500 system 10 ngml. Go to api instrument folder and open the acquisition method for calibration. This video demonstrates how to acquire data in manual tune mode in analyst software, using ppg tuning as an example. The proven quadrupole quantitation capabilities of the api 4000. This manual contains the instructions required to start up and operate the components of the. Ab sciex api 5000 lcms ms manuals manuals and user guides for ab sciex api 5000 lcms ms. Installation and one year service contract pm at 6 months method development and training also available. Enhanced high performance triple quadrupole mass spectrometer system. Select a new project from the project list and then. The proven quadrupole quantitation capabilities of the api 4000 instrument with additional scan types. Anyone know about hardware problems with a 4000 qtrap mass. View online or download ab sciex 4000 series site planning manual. The api 4000 lcmsms system is premier hybrid triple quadrupolelinear ion trap mass spectrometer.

Operation procedure for ab sciex api 30004500 by jianjun. Quantitative analysis of small molecules qualitative analysis of small molecules qualitative analysis of proteins and peptides quantitative analysis of small molecules this application involves measurement of specific molecular weight compounds. The picture is of the 5800 toftof table of contents to illustrate the quality of these files. Enhanced ms, enhanced multiply charged, enhanced product ion, enhanced resolution, msmsms, and. Ab sciex api 4000 qtrap w shimadzu 20ad uplc lcmsms q trap system. No matter your skill level, this 4000 system takes clinical samples, pesticide and drug residues, toxicology. The 4000 lcmsms system is a hybrid triple quadrupolelinear ion trap mass spectrometer. Ab sciex api 5000 lcmsms hardware manual pdf download. The ab sciex api 4000 lcms system brings you quick and accurate identification across a broad range of lc flow rates with high performance quantitation. Ab sciex api 4000 qtrap w agilent 1100 hplc lcmsms q.

Features of the instrument the api 5000 lcmsms system combines all of the features of the api 4000 lcmsms system with the additional benefits of improved sensitivity and enhanced signalto. Ideal for drug discovery and development, metabolite identification, contaminant testing, and proteomic applications including posttranslationally modified proteins discovery, protein identification, and biomarker validation. Laboratory equipment ab sciex 4000 qtrap hardware manual 18 pages laboratory equipment ab sciex 4000 series site planning manual 33 pages. Operation procedure for ab sciex api 30004500 by jianjun chen updated by dejian ma, jan 2015 contact info. Class 2 device recall ab sciex api 3200md, 3200md qtrap. The 4000 qtrap system offers the following benefits over a standard triple quadrupole and a traditional 3d ion trap mass spectrometer. Applied biosystems 4000 qtrap inquire about availability applied biosystems mda sciex 4000 q trap lcmsms system, with shimadzu hplc system hplc is shimadzu dgu20a5 degasser, shimadzu lc20ab pumps, shimadzu lc20ad pump, shimadzu cbm20a controller, shimadzu spd10a uvvis detector including ctc pal autosampler, computer with analyst. If your instrument has an integrated syringe pump, in the method list field, click. View online or download ab sciex 4000 qtrap hardware manual. Sciex api 4000 qtrap mass spectrometer atlantic lab. This manual is not designed to instruct operators on procedures for the. Download download api 3200 manual read online read online api 3200 manual api 4000 installation guide sciex api 5000 ab sciex 4000 qtrap api 3000 service manual api 3200 lcmsms system api 4000 manual ab sciex 4000 qtrap manual analyst software manual high performance, higher productivity, greater value. The system is ideal for drug discovery and development, metabolite identification, contaminant testing, and proteomic applications including posttranslationally modified protein discovery, protein identification, and biomarker validation.

Uv, fluorescence and radiometric detection in conjunction with mass spectrometry. The api 4000 qtrap lcmsms system employs hybrid triplequadrupole and linear ion trap technologies to analyze a wide range of molecule sizes and sample types. For safety information, refer to the system user guide or the safety practices guide. Manual optimization is used to optimize compound and ion sourcedependent parameters for the analyte. Ideal for drug discovery and development, metabolite 4000 qtrap manual system 4000 qtrap manual though the results are solid for singleimage projects, it could be even better if you could. On the navigation bar, under tune and calibrate, doubleclick manual tuning. Anyone know about hardware problems with a 4000 qtrap mass spectrometer. Double click little green quadrupole icon in the bottomright corner to invoke q trap. Descriptionthe sciex api 4000 qtrap mass spectrometer system delivers highperformance quantitation and identification of small molecules, peptides, and proteins across a wide range of lc flow rates. August 2011 7 foreword this guide is for instrument operators who are new to the analyst software. Infuse 5 to 10 lmin of either ppg solution for tuning q1 and q3 in positive mode, or ppg 3000, solution for tuning in q1 and q3 negative mode.

Class 2 device recall ab sciex api 3200md, 3200md qtrap, triple quad, qtrap 4500md lcmsms systems. When you manually optimize for an analyte, you create an ms acquisition method in. Api 3200 system and 3200 qtrap system 50 ngml api 4000 system and 4000 qtrap system 10 ngml api 5000 system 10 ngml ab sciex qtrap 5500 system 10. And because this system can trigger msms and ms 3 from mrm transitions, you can confirm the presence and structure of metabolites at the same time. This manual contains the instructions required to start up and operate the components of the api 4000 system. Sciex api 4000 qtrap lcmsms, with agilent 1100 system and nitrogen generator.