The antioxidative activity of a total of 92 phenolic extracts from edible and nonedible plant materials berries, fruits, vegetables, herbs, cereals, tree materials, plant sprouts, and seeds was examined by autoxidation of methyl linoleate. Antimicrobial and antioxidant activity of leaf and flower. Antioxidant activity of polyphenolic plant extracts by dimitrios stagos department of biochemistry and biotechnology, university of thessaly, 41500 biopolis, greece. Antioxidant activity of various tea extracts in relation. Jan 14, 2002 three methods widely employed in the evaluation of antioxidant activity, namely 2,2diphenyl. Comparatively, methanolic extract showed higher activity than ethanolic activity. Antioxidant activity of some common medicinal plants. A comparative study on the antioxidant activity of. The significant linear correlation was confirmed between the values for the total phenolic content and antioxidant activity of plant extracts. Antioxidant and antilipid peroxidation activities of. Screening of medicinal plant extracts for antioxidant activity.
Evaluation of the in vitro and in vivo antioxidant. The extracts were investigated for the antioxidant activity using 2, 2 diphenyl, 1 picryl hydrazyl. The present study evaluated the antioxidant effects of methanolic seed extract of a. Screening of in vitro antioxidant activity of methanolic leaf and root extracts of hypochaeris radicata l.
The content of total phenolics in the extracts was determined spectrometrically according to the folin. Pdf evaluation of antioxidant activity of medicinal plant extracts. Evaluation of antioxidant activity of some plant extracts. Screening of in vitro antioxidant activity of methanolic. Evaluation of antioxidant activity of medicinal plant. The antioxidant activities of methanol extracts of oregano, dittany, thyme, marjoram, spearmint, lavender and basil were tested in lard stored at 75c. Aug 19, 2015 the antioxidant activity of plant extracts is not limited to phenolics javanmardi et al. In vitro nitric oxide scavenging activity of methanol.
Antioxidant properties of extracts from selected plant materials. Comparative analysis of the antioxidant activity of cassia. Mar 16, 2001 based on our results, we can say that as a general rule the ethanol extracts of plants belonging to the verbenaceae family showed lower ec 50 values than the other plant extracts. Antioxidant properties of several medicinal plants growing. The results indicated that both leaves and seed extracts have an antioxidant activity. The effect of various plant extracts was measured on several important oxidation indicators. The many number of medicinal plants are used in the cellular and metabolic. Antioxidant activity dpph free radical scavenging activity of methanolic extract the antioxidant activity of the plant extracts and the standard was assessed on the basis of the radical scavenging effect of the stable 1, 1diphenyl2picrylhydrazyl dpphfree radical activity by modified method. The concentration of extracts in lard varied from 0.
Determination of antioxidant activity the antioxidant activity of plant extracts against peroxidation of linoleic acid was determined by the reported method 16. Herbal plants considered as good antioxidant since ancient times. Antioxidant activity, total phenolic and total flavonoid. Hence, in this study prepawe red polyphenol extracts from papaya peel pp with the aim of exploiting potential antioxidant and anticancer properties of pp extracts. Four complementary methods were used to determine the antioxidant activity of methanolic extracts. Materials and methods fresh mature papaya fruits variety caribbean red were obtained from a local health food store, huntsville, al. The aim of this study was to screen various solvent extracts of whole plant of torilis leptophylla to display potent antioxidant activity in vitro and in vivo, total phenolic and flavonoid contents in order to find possible sources for future novel antioxidants in food and pharmaceutical formulations. The antioxidant activities were determined by total antioxidant capacity, dpph 1,1diphenyl2picrylhydrazine radical scavenging assay, hydroxyl radical scavenging assay, ferrous reducing antioxidant capacity and lipid peroxidation inhibition assay methods. Superoxide radical scavenging activity data represents, mean sem, for n3 figure 3. However, the total antioxidant activities of plant extracts cannot be evaluated by using one single method, due to the complex composition of phytochemicals as well as of oxidative processes. The antioxidant activity of extracts was evaluated according to the amount of malonaldehyde mda formed by the feso 4induced oxidation of linoleic acid and a high pufa oil. Screening of antioxidant activity and phenolic content of. Antioxidant properties of the methanol extracts of the leaves.
Evaluation of antioxidant capacity of plant extracts by. The present study reveals that extracts are a good source of antioxidant property containg phytoconstituents. Screening of plant extracts for antioxidant activity. Therefore, the aim of this research was to evaluate the antioxidant activity of e. Antioxidant activity determination of citronellal and. Frap, dpph and total phenols by the folincitocalteau electron transfer methods and abts a method.
Antioxidant activity of some plant extracts of the family. Extraction and determination of antioxidant activity of. Antioxidant activity of plant extracts containing phenolic. Fruit extracts for total phenolics and antioxidant activity measured in methanol extract aoam analysis were prepared using the method of swain and hillis 1959, with some modi. Therefore, plant oils are widely used as a substrate for testing antioxidant activity. The antioxidant activity may also contribute from the other antioxidant secondary metabolites such as volatile oils, carotenoids and vitamins wongsa et al. The leaves extracts show a higher activity on cat and asa peroxidase activity, while the. The most common methods involve the determination of the ability to scavenge free radicals using dpph assay, ferric reducing antioxidant power frap assay and ferrousion chelating assay singh and rajini, 2004. Preliminary phytochemical screening, quantitative analysis. Researcharticle preliminary phytochemical screening, quantitative analysis of alkaloids, and antioxidant activity of crude plant extracts from ephedra intermedia indigenous to balochistan rahmangul,1,2,3 syedumerjan,1,4 syedfaridullah,3 samiullahsherani,1 andnusratjahan3 1facultyofpharmacy,universityofbalochistan,quetta,pakistan 2departmentofhealth,governmentofbalochistan,quetta,pakistan. Screening of brazilian plant extracts for antioxidant. Three methods widely employed in the evaluation of antioxidant activity, namely 2,2diphenyl1 picrylhydrazyl dpph radical scavenging method, static. The antioxidant activity and phenolic contents of the stem as determined by the dpph, proanthocyanidins, total. Phytochemical screening and antioxidant activity of moroccan.
Antioxidant activity, active nitrogen species, sodium nitroprusside. Antioxidant activity of polyphenolic plant extracts mdpi. Phytochemical screening and evaluation of antioxidant and. Pdf different extracts from five medicinal plants in terms of phenolic compounds and antioxidant properties were studied. The antioxidant activity of plant extracts is not limited to phenolics javanmardi et al. In the previous chapters 3 and 4, it was shown that p. Antioxidant activity of the leaf extract was assessed by dpph, cuprac and pfrap assays using lascorbic acid as standard. Antioxidant activity of methanol extracts of different parts of lantana. The high contents of phenolic compounds indicated that these compounds contribute to the antioxidant activity. In vitro nitric oxide scavenging activity of methanol extracts of three bangladeshi medicinal. The antioxidant activities of the methanol extracts from the leaves and stems of celtis africana ulmaceae were assessed in an effort to validate the medicinal potential of the subterranean part of the herb. Antioxidant properties of plant extracts edelweiss publications.
Libyan capparis spinose has good antioxidant activity. Screening of plant extracts for antioxidant activity wiley online. Extract, free radical, antioxidant activity, medicinal plant. Therefore, in this study was tested in vitro using cape method to see the antioxidant activity of ethanol extract in normal red blood cells, red blood cells of. Evaluation of antioxidant activity of medicinal plant 885 figure 2. Antioxidant activity of plant extracts and their effect on. Antioxidant activity of polyphenolic plant extracts. To observe the antioxidant activity of leaves and seed extracts of n. Antioxidant activity was assessed by using 2,2diphenyl1picrylhydrazyl dpph assay, reducing power activity, 2,2azinobis3. In the present paper we have investigated antioxidant activity of extracts from withania somnifera dunal. In the present study, three plant foods, namely, drumstick leaves moringa oleifera, mint leaves mentha spicata and carrot tuber daucus carota were extracted with ethanol and analyzed for their antioxidant activity. Furthermore, it was estimated that more than 50% of prescribed drugs are either directly derived from herbsplants or active ingredients obtained from plant. All plant extracts, except for tarragon, retarded peroxide formation at 80c the most effective being sage and sweet grass extracts fig.
Ethiopia is one of the developing countries which have enormous diversity of plants. Antioxidant activity and phenolic content of some medicinal. From this study it is observed that all the plants posses marked antioxidant effect. Among the partitions, the more polar ones ethyl acetate and n. Pdf methods for determining the antioxidant activity. Oregano extract was found to be the most effective in stabilizing lard, followed by thyme, dittany, marjoram and lavender extracts, in a decreasing order. Dpph free radical scavenging activity of the extracts of. Ferric reducing antioxidant potential assay frap assay the frap assay was employed to estimate the antioxidant capacity of the samples in vitro. The scavenging activity of the plant extract through the annihilation of the dpph radicals was investigated. Abstract identification and characterization of natural products with antioxidant and antiproliferative activity has received much interest over the past few years.
Three grams of guava tissue were mixed with 25ml methanol and homogenized using the ultraturrax homogenizer. Pdf the antioxidant potential of some herbal plant extracts commercial products was measured using various in vitro assays. A detailed study was performed on the antioxidant activity of the methanol extract of whole. Antioxidant and apoptotic activity of papaya peel extracts.
The antioxidant effects were evaluated using in vitro, 2, 2diphenylpicrylhydrazine photometric assay and in vivo serum catalase, superoxide dismutase and. Among the important biological properties exhibited by plant polyphenols, their antioxidant activity has raised a great interest 1. The lethal dose of the methanolic extract of the plant leaves is more than 2000mgkg. Antioxidant activity from perepat plant sonneratia alba. This result identifies this plant as a good candidate for further investigations. Pdf in vitro antioxidant and antiproliferative activity. Karthika abstract the aim of present study was to estimate the total phenolic and flavonoid contents in vitro antioxidant potential of methanolic leaf and root extracts of the herb. Phytochemical screening and antioxidant activity of ethanol extract. For a typical assay an aliquot of 20 l of three dilutions of each extract in ethanol 0.
Gc and carotene bleaching test bcbt, have been compared with regard to their application in the screening of plant extracts. Aframomum melegueta schum zingiberaceae is a perennial herb widely cultivated for its valuable seeds in the tropical region of africa. Among the extracts, tsb showed the highest antioxidant activity followed by trb, tf. Methanolic extracts of cassia fistula showed the highest amount of phenolic and flavonoid content and reducing capacity, whereas hexane extracts exhibited the lowest level of reducing capacity. Total antioxidant activity and phenols and flavonoids content of several plant extracts claudiavalentina popa1, liliana lungu 2, manuela savoiu, corina bradu1, vasile dinoiu2, and andrei florin danet1 1faculty of chemistry, university of bucharest, bucharest, romania 2institute of organic chemistry c. Original article comparison of abts, dpph, frap, and orac. Total phenols were estimated by folinciocalteus method and flavonoids by. The antioxidant activity of the plant extracts was examined on the basis of the scavenging effect on the stable dpph free radical activity braca et al. Evaluation of antioxidant activity of medicinal plant extracts.
69 262 687 862 163 1178 1417 124 1350 58 1165 521 404 1308 870 522 1246 1584 854 1055 198 921 1145 524 1112 73 826 621 238 867 716 783 437